Giardia-like microorganisms were found as a result of a Cryptosporidium/Giardia detection assay on winter reservoir samples. The morphological aspects, DNA sequence of the microorganism, and specificity of immunoassay reagents were investigated. The microorganism was very similar to a Giardia cyst. The cell was oval shaped (11.0 μm in length, 7.7 μm in width), contained organelles and was stained bright apple-green by a fluorescent monoclonal antibody, although no axonemes or curved bristles were observed. Cross-reaction occurred with the anti-Cryptosporidium, but not the anti-Giardia, immunomagnetic antibody. The specificity of the fluorescent monoclonal antibodies differed depending on the product. As a result of the analysis of the partial sequence of 18S rDNA, the microorganism was identified as Lunulospora curvula, mitosporic Ascomycota. These findings will be very helpful for avoiding false positives in future assays.
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Research Article|
August 01 2012
Characterization of a Giardia-like microorganism causing false positive reactions in the detection of Cryptosporidium/Giardia from water samples Available to Purchase
Taketoshi Shimizu;
1Water Quality Laboratory, Engineering Department, Kobe City Waterworks Bureau, 37-1 Kusutani-cho, Hyogo-ku, Kobe 652-0004, Japan
E-mail: [email protected]
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Takuya Oda;
Takuya Oda
1Water Quality Laboratory, Engineering Department, Kobe City Waterworks Bureau, 37-1 Kusutani-cho, Hyogo-ku, Kobe 652-0004, Japan
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Hiroyuki Ito;
Hiroyuki Ito
1Water Quality Laboratory, Engineering Department, Kobe City Waterworks Bureau, 37-1 Kusutani-cho, Hyogo-ku, Kobe 652-0004, Japan
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Shoji Uga
Shoji Uga
2Department of Parasitology, Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan
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Water Supply (2012) 12 (5): 604–610.
Article history
Received:
February 27 2012
Accepted:
March 29 2012
Citation
Taketoshi Shimizu, Takuya Oda, Hiroyuki Ito, Shoji Uga; Characterization of a Giardia-like microorganism causing false positive reactions in the detection of Cryptosporidium/Giardia from water samples. Water Supply 1 August 2012; 12 (5): 604–610. doi: https://doi.org/10.2166/ws.2012.032
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