Arsenic contamination in groundwater has caused severe health problems throughout the world. Developing cost-effective processes for arsenic removal is an emerging issue. Because As(III) is predominant in groundwater and is more difficult to remove than As(V) is, oxidation of As(III) to As(V) is necessary to improve overall arsenic removal. This study was undertaken to enrich arsenite oxidizing bacteria under autotrophic conditions and to isolate and characterize facultative chemolithoautotrophic arsenite oxidizing bacteria (CAOs) that can oxidize As(III) effectively to As(V). An enrichment culture which adapted wide As(III) concentrations and completely oxidized 12 mM As(III) within 4 days under autotrophic conditions was established and maintained. Among 10 isolated strains, 6 strains, B1, B2, C, D, E1 and E2 belonging to β-Proteobacteria, were facultative CAOs and contained aoxB genes encoding the arsenite oxidase large subunit. Furthermore, they displayed various As(III) oxidation capabilities: B1, B2, E1 and E2 efficiently oxidized 1–10 mM As(III). The others showed efficient oxidation at 1–5 mM As(III), suggesting the coexistence of facultative CAOs with various As(III) oxidation capabilities in the enrichment. These results suggest that constructed enrichment and strains B1, B2, E1 and E2 can be useful for the bioremediation of arsenic-contaminated groundwater.
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Research Article|
August 01 2012
Enrichment of arsenite oxidizing bacteria under autotrophic conditions and the isolation and characterization of facultative chemolithoautotrophic arsenite oxidizing bacteria for removal of arsenic from groundwater Available to Purchase
L. Nguyen Ai;
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
E-mail: [email protected]
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A. Sato;
A. Sato
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
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D. Inoue;
D. Inoue
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
2Department of Health Science, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan
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K. Sei;
K. Sei
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
2Department of Health Science, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan
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S. Soda;
S. Soda
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
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M. Ike
M. Ike
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565–0871, Japan
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Water Supply (2012) 12 (5): 707–714.
Article history
Received:
February 17 2012
Accepted:
April 24 2012
Citation
L. Nguyen Ai, A. Sato, D. Inoue, K. Sei, S. Soda, M. Ike; Enrichment of arsenite oxidizing bacteria under autotrophic conditions and the isolation and characterization of facultative chemolithoautotrophic arsenite oxidizing bacteria for removal of arsenic from groundwater. Water Supply 1 August 2012; 12 (5): 707–714. doi: https://doi.org/10.2166/ws.2012.045
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