This article presents a methodology developed in order to identify the potential by-products of emerging chemical pollutants generated during oxidation treatments like chlorination and ozonation. As an example, the study of ozonated real river water samples spiked with estrone sulfate (E1-S) was used to evaluate the impact of ozonation on E1-S concentration and to identify the generated treatment by-products (TBPs). After a large-scale solid phase extraction, samples were analyzed by liquid chromatography coupled with high resolution mass spectrometry (HRMS) and data were processed according to a differential metabolomic-like approach to uncover the produced compounds. Four main TBPs were found. ERα-CALUX bioassays were performed to measure the estrogenic potency of samples before and after treatment. Results show that the estrogenic potency decreases drastically in drinking water with ozonation.

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