Pseudomonas aeruginosa, a food- and water-borne opportunistic pathogen, constitutes a health risk mostly to immunocompromised patients, and also affects the taste, odour and turbidity of potable water. In order to detect P. aeruginosa in bottled water in Sri Lanka, 36 bottled water brands were collected randomly from retail and supermarkets island wide. P. aeruginosa was detected by the membrane filtration technique, using cetrimide agar supplemented with nalidixic acid. The isolates were subjected to confirmatory tests, viz: ISO 16266:2006 methods and growth at 4 and 42°C, and API 20NE followed by DNA sequencing. Presumptive isolates of P. aeruginosa were observed on cetrimide agar in 50% of brands. Among these isolates, ISO procedures confirmed 58% as P. aeruginosa. Thirty-nine randomly selected isolates were identified as P. aeruginosa by the API 20NE, three of which were further confirmed by DNA sequencing. The presence of P. aeruginosa in bottled water raises health concerns since P. aeruginosa is an indicator of inferior water quality. Therefore, strict regulations and regular monitoring of bottling plants are recommended in order to supply safe and acceptable bottled drinking water to the Sri Lankan market.
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Research Article|
June 13 2014
Pseudomonas aeruginosa in bottled drinking water in Sri Lanka: a potential health hazard Available to Purchase
A. T. Herath;
1Department of Botany, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
E-mail: [email protected]
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C. L. Abayasekara;
C. L. Abayasekara
1Department of Botany, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
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Rohana Chandrajith;
Rohana Chandrajith
2Department of Geology, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
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N. K. B. Adikaram
N. K. B. Adikaram
1Department of Botany, Faculty of Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
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Water Supply (2014) 14 (6): 1045–1050.
Article history
Received:
March 03 2014
Accepted:
May 12 2014
Citation
A. T. Herath, C. L. Abayasekara, Rohana Chandrajith, N. K. B. Adikaram; Pseudomonas aeruginosa in bottled drinking water in Sri Lanka: a potential health hazard. Water Supply 1 December 2014; 14 (6): 1045–1050. doi: https://doi.org/10.2166/ws.2014.057
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