Fitting a pre-established region-specific Parallel Factor Analysis (PARAFAC) model to new dissolved organic matter (DOM) samples has become a popular approach in DOM studies. A key step during the development of the pre-established model is to identify the fluorescence fingerprint, i.e. the number of fluorescent DOM (FDOM) components as well as their excitation and emission spectral features. In practice the samples to use for model development may not be measured immediately after sampling and will instead be stored for a relatively long time, thus raising the question whether the storage would change the intrinsic fingerprints. In this study, two PARAFAC models were separately developed and validated for the same set of surface water DOM samples from an estuary and its river, immediately after sampling and after 9-month storage respectively. The fingerprint did not change after storage, regardless of the change of the intensity of some components. The findings in this study highlighted that DOM samples stored using a simple protocol (i.e. filtration to 0.45 μm pore size without any preservatives and placed at 4 °C in the dark under airtight conditions) for a few months would not change fluorescence fingerprints for PARAFAC and broaden our understanding of the application of PARAFAC to DOM studies.
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Research Article|
July 23 2015
Long-term effect of a simple storage protocol on fluorescence fingerprint of dissolved organic matter Available to Purchase
Xuechun Wang;
Xuechun Wang
1College of Chemistry and Chemical Engineering, Inner Mongolia University, 235 West University Road, Hohhot 010021, China
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Hao Chen;
2State Environmental Protection Key Laboratory in Coastal and Estuarine Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
E-mail: [email protected]
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Kun Lei;
Kun Lei
2State Environmental Protection Key Laboratory in Coastal and Estuarine Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
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Zhong Sun
Zhong Sun
1College of Chemistry and Chemical Engineering, Inner Mongolia University, 235 West University Road, Hohhot 010021, China
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Water Supply (2016) 16 (1): 44–51.
Article history
Received:
December 15 2014
Accepted:
July 10 2015
Citation
Xuechun Wang, Hao Chen, Kun Lei, Zhong Sun; Long-term effect of a simple storage protocol on fluorescence fingerprint of dissolved organic matter. Water Supply 1 February 2016; 16 (1): 44–51. doi: https://doi.org/10.2166/ws.2015.111
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