The present study aimed to evaluate the stability of Human Adenovirus type 2 (HAdV2) and Murine Norovirus 1 (MNV-1) in surface freshwater samples stored at different temperatures. For HAdV2 the stability decreased with increasing temperatures (−80 > −20 > 4 > 22 °C). The time required to reach one log reduction in viral titers (T90) was similar among all the times and temperatures by different cell-culture based methods and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The HAdV2 stability decreased with the time of storage temperature and methods employed, aside from samples stored at 22 and 4 °C which showed the lowest T90 values (50 days). For MNV-1, the samples stored at 22 and −20 °C showed higher log10 decay values, followed by 4 and −80 °C; while genome persistence was ranked as −80 > −20 > 4 > 22 °C. The T90 values were lower for samples stored at 22 °C (33 days), followed by 4, −20 and −80 °C with 111, 100 and 333 days, respectively. The results indicate that, under laboratory storage conditions, freshwater samples should be kept at 4 °C and at −80 °C for short- and long-term periods, respectively. This study provided useful information about thermal and temporal stability of the enteric viruses regarding sample storage conditions.
Thermal and temporal stability on the enteric viruses infectivity in surface freshwater
V. Moresco, N. A. Damazo, C. R. M. Barardi; Thermal and temporal stability on the enteric viruses infectivity in surface freshwater. Water Science and Technology: Water Supply 1 June 2016; 16 (3): 620–627. doi: https://doi.org/10.2166/ws.2015.171
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