Microbially available phosphorus (MAP) is the labile phosphorus that is readily assimilated by microorganisms, which is linearly correlated to bacterial re-growth in drinking water in some regions. The conventional MAP bioassay for drinking water was originally developed by Markku based on the growth potential of Pseudomonas fluorescens P17 (P17). However, the bioassay bears some demerits, such as time-consuming and labor-intensive enumeration. For convenience, an alternative method based on a similar principle was developed to assess the content of MAP in drinking water, in which natural microbial consortium was used as inoculum instead of pure culture P17, cell number was counted using flow cytometry (FCM), and cultivation at 30 °C was adopted. Natural microbial consortium is able to efficiently utilize organic phosphorus and exhibit high sensitivity since more cells are produced per μg P utilized. FCM is a rapid method to count all bacteria growing in drinking water. With incubation temperature increasing up to 30 °C, there is a shorter test period (64 h), excellent sensitivity and better utilization efficiency for organic phosphorus. The results show that the developed bioassay is sensitive, time-saving and easily operated.
Skip Nav Destination
Article navigation
Research Article|
March 15 2016
An improved method for determining microbially available phosphorus in drinking water Available to Purchase
Gang Wen;
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
2State Key Laboratory Cultivation Base of Architecture and Technology in West China, Xi'an University of Architecture and Technology, Xi'an 710055, China
E-mail: [email protected]
Search for other works by this author on:
Qin Deng;
Qin Deng
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Search for other works by this author on:
Ting-Lin Huang;
Ting-Lin Huang
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
2State Key Laboratory Cultivation Base of Architecture and Technology in West China, Xi'an University of Architecture and Technology, Xi'an 710055, China
Search for other works by this author on:
Jun Ma
Jun Ma
3State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
Search for other works by this author on:
Water Supply (2016) 16 (4): 1149–1158.
Article history
Received:
August 20 2015
Accepted:
March 02 2016
Citation
Gang Wen, Qin Deng, Ting-Lin Huang, Jun Ma; An improved method for determining microbially available phosphorus in drinking water. Water Supply 1 August 2016; 16 (4): 1149–1158. doi: https://doi.org/10.2166/ws.2016.036
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
eBook
Pay-Per-View Access
$38.00