Invertebrate removal by traditional biological activated carbon (tra-BAC) and pre-BAC treatment processes was investigated in a full-scale water treatment plant. The results showed that invertebrate reproduction occurred in both BAC filters, but the invertebrate abundance in the finished water processed by tra-BAC was about 15 times greater than that processed using the pre-BAC process. In the pre-BAC process, the sand filter was placed after the BAC filter, and sand filtration removed most of the invertebrates, with an average removal efficiency of 91.1%. However, the pre-BAC filter, which was positioned behind the sedimentation tank, needed to be backwashed more frequently than the tra-BAC filter because of the high turbidity of the inlet water. The frequent backwashing reduced the biomass on the activated carbon and decreased the invertebrate reproductive rate. The results of this study are helpful for evaluating the pre-BAC treatment process in drinking water treatment plants.