The desorption behavior of ammonia nitrogen (NH3-N) in cellar sediment was studied to illustrate the influence of sediment on the quality of water in cellar rain collection. The impacts of three factors on the ability of cellar sediment to desorb NH3-N were analyzed, which include the concentration of the cellar sediment, temperature and the degree of disturbance. In addition, the isothermal balance and kinetics fitting were studied. The results show that sediment concentration, temperature, and the disturbance degree greatly affect the NH3-N desorption process. The pseudo-first-order and pseudo-second-order kinetics equations are suitable for describing the desorption process (R2 > 0.86) under the different conditions of cellar sediment concentration. The Langmuir isothermal model is more suitable for describing the equilibrium desorption of the different sediment concentrations than the Freundlich isothermal model. Ultimately, the NH3-N desorption process has a significant influence on cellar water quality. Such results could be a theoretical basis for collection, treatment, and maintenance of cellar water.