This study assessed the occurrence and prevalence of antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs) in tap water sampled across London, United Kingdom. Sampling was conducted seasonally from nine locations spread geographically across the city. ARBs and ARGs (tet(A), dfrA7, and sul1) were detected in all sampling locations in all sampling rounds. Resistance to trimethoprim was the highest among the tested antibiotics and the sul1 gene was the most abundant resistance gene detected. Several opportunistic pathogens were identified amongst the ARBs in the water samples, including Pseudomonas aeruginosa and Stenotrophomonas maltophilia.