Optimizing hydropower plants is complex due to nonlinearity, complexity, and multidimensionality. This study introduces and evaluates the performance of the Wolf Search Algorithm (WSA) for optimizing the operation of a four-reservoir system and a single hydropower system in Iran. Results indicate WSA could reach 99.95 and 99.91 percent of the global optimum for the four-reservoir system and single reservoir system, respectively. Comparing the results of WSA with a Genetic Algorithm (GA) also indicates WSA's supremacy over GA. Thus, due to its simple structure and high capability, WSA is recommended for use in other water resources management problems.