Owing to frequent overflow of sewage-polluted creeks and rivers in São Paulo city, the population that lives in those surrounding areas is constantly exposed to a high risk of infection by many pathogens. Our aim was to evaluate the occurrence of human adenovirus, especially F species (HAdV-40 and HAdV-41), in sewage and creek water in São Paulo city. A total of 95 water samples were collected from July 1998 to June 1999 and from September 2000 to December 2001 at Sewage Pumping Station Edu Chaves and Creek Pirajussara. Four-litre samples were concentrated by a two-step procedure based on filtration through electropositive filter membrane and ultracentrifugation. Adenoviruses were detected in 69 samples (72.6%) after amplification of a sequence of the hexon gene by PCR. The discrimination of human adenovirus F was done by RFLP. A first screening was performed by restriction of PCR product with TaqI, followed by the discrimination of HAdV-40 and HAdV-41 by RsaI restriction. Sixty-two (89.8%) samples showed F species viruses. Among them, human adenovirus, serotype 41, was the most frequently detected in 17 (62.9%) out of 27 samples and serotype 40 was identified only in 7 (25.9%) samples. No seasonal distribution of adenoviruses was observed.
Discrimination of adenovirus types circulating in urban sewage and surface polluted waters in São Paulo city, Brazil
F.M. Santos, M.J. Vieira, P. Garrafa, T.A. Monezi, V.H. Pellizari, C.M. Hársi, D.U. Mehnert; Discrimination of adenovirus types circulating in urban sewage and surface polluted waters in São Paulo city, Brazil. Water Science and Technology: Water Supply 1 April 2004; 4 (2): 79–85. doi: https://doi.org/10.2166/ws.2004.0031
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