Abstract

As a kind of odorous substance, 2-methylisoborneol (2-MIB) is difficult to be degraded naturally. Some isolated strains of bacteria could degrade 2-MIB effectively. In this study, a strain of bacteria which can remove 2-MIB from drinking water efficiently was obtained from activated carbon in filter, and was identified to be Pseudomonas mandelii based on 16S rRNA gene sequence analysis. Pseudomonas mandelii was not sensitive to the initial concentration of 2-MIB, and could tolerate a rather high concentration of 2-MIB. The best growth condition for this degrader was 25–35 °C and initial pH of 7. The concentration of 2-MIB in mineral salts medium was reduced from 2 mg/L to 471.9 μg/L by Pseudomonas mandelii in 20 d after incubation. Nineteen bands of degrading enzyme were isolated from Pseudomonas mandelii, one of which was identified as a NAD-dependent dehydratase. 2-Methyl-2-bornene was found to be the metabolites in the presence of both the Pseudomonas mandelii and the isolated enzymes, indicating that NAD-dependent dehydratase might be involved in the biodegradation process or cooperate with other enzymes in the metabolic process to complete the dehydration process of 2-MIB.

HIGHLIGHTS

  • Pseudomonas mandelii was reported to be 2-MIB degrader.

  • Degrading enzymes were isolated from Pseudomonas mandelii.

  • NAD-dependent dehydratase might help to complete the 2-MIB dehydration process.

  • 2-Methyl-2-bornene was found to be the metabolites in the presence of Pseudomonas mandelii or the isolated enzymes.

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