We investigated the interaction of As(III) with As(III)-binding DNA aptamers (Ars-3). The binding of Ars-3 to As(III) was investigated using a Ars-3 sequence and a few control DNA sequences. As a result, we conclude that Ars-3 cannot bind As(III) and As(III) can adsorb onto gold nanoparticles with selectivity. However, the As(III) concentration could be determined by a simple As(III) analysis method using Ars-3. And we measured the As(III) and As(V) concentration separately in As(III)/As(V) mixed samples in ultrapure water and groundwater using a simple analytical method for As(III) and As(V), respectively. As(III) in the sample was measured by a simple As(III) analytical method using DNA aptamers and gold nanoparticles, and As(V) in the sample was measured by a simple As(V) analysis method using cerium oxide nanoparticles and fluorescence-labeled DNA. We can measure As(III) and As(V) concentrations in ultrapure water and groundwater in the simple analytical method for As(III) and As(V), separately.

  • We investigated the interaction between Ars-3 and As(III).

  • We determined As(III) and As(V) concentrations by simple analytical method for As(III) and As(V).

  • We hypothesized that the simple As(III) analysis method can quantify As(III) concentration by the adsorption of As(III) and ssDNA onto AuNPs.

  • We could determine the As(III) concentration and As(V) concentration in As(III)/As(V) mixed samples for each chemical species.

Graphical Abstract

Graphical Abstract
Graphical Abstract
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