Removal of poliovirus 1 by activated sludge treatment in batch experiments was investigated, virus counts were reduced by more than 99% under conditions of 0.2 to 0.4 kg MLSS−−1 and MLSS concentrations of 1,000 to 2,000 mg.l−1. There were no distinct relationships between BOD-SS loadings or TOC of the effluent and virus inactivation rates. MLSS levels of more than 800 mg.l−1 were necessary for more than 99% reduction in viral counts. Although MLSS levels of more than 2,000 mg.l−1 reduced viral counts by 4 log values, factors such as low temperatures and anaerobic conditions which adversely affect the efficiency of activated sludge processes, resulted in prolonged virus survival.

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