Experimental results with removal of soluble substrates in fixed films are presented. Experiments are performed with denitrification and with oxidation of methanol, acetic acid and glucose in a laboratory fixed film reactor.

The experiments confirm the predictions of the half order reaction model, taking diffusion of the substrates and zero order intrinsic reaction rate of the bacteria as the dominating processes in the film. The experiments enable calculation of the kinetic parameters: diffusion coefficients, intrinsic reaction rates and half order rate constants for nitrate, methanol, acetic acid, glucose and oxygen within fixed films.

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