The authors attempted to extract the floc-forming substances by sequential extraction with NH4OH and EDTA solutions from activated sludge. Some fractions had the capacity for floc formation in acidic ranges and some of the fractions were further fractionated by Sephadex G-50. There is a possibility that low molecular weight substances, except metallic ions related to floc formation and floc-forming substances in activated sludge are not limited to one special substance.

aerobic sewage treatment, activated sludge, flocculation, floc-forming substances, organic substances, metallic ions
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