An enzyme calorimetric method, employing immobilized cholinesterase to detect harmful levels of organophosphorus and carbamate insecticides in water, is described.

Immobilized cholinesterase activity was assessed automatically in a microcalorimeter by analyzing its catalytic efficiency in hydrolyzing a substrate solution which was passed over the enzyme at regular time intervals. A microcomputer was used to automate the procedure and for data acquisition.

The inhibitory effects of three organophosphorus and two carbamate pesticides upon cholinesterase were studied in order to evaluate the efficacy of the technique.

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