Anaerobic treatment of sewage has a problem of denitrification, because that organic carbon and hydrogen are recoverd mainly in the form of methane and not used for denitrification. Other electron donors must be introduced. Promising donors are reduced forms of sulphur. In this study, thiosulphate and elemental sulphur were used in fluidized bed reactor systems. Denitrification by oxidation of sulphur was unstable due to poor solubility of sulphur into sewage. Thiosulphate denitrification was very stable. Stoichiometric relationship of the denitrification and thiosulphate oxidation for the reactor was obtained. The denitrification process was described by the first order consecutive reactions. The rate constant of nitrate to nitrite reduction, kl varied between 2.05×10−2 and 1.09×10−1 (1/gVSS/l*min.). The rate constant of nitrite to nitrogen reduction, k2 varied between 1.41×10−1 and 9.08×10−1 (l/gVSS/l*min.).

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