The effect of segregation of different species of participate material (e.g. microorganisms) on biofilm performance is discussed with the aid of analytical and numerical model predictions. Based on two case studies it appears that segregation is an important aspect of biofilm behaviour and cannot a priori be neglected as is frequently the case in biofilm modelling. In order to improve the reliability of deterministic biofilm models, experiments are required to define the significance of segregation in biofilms.

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