Anaerobic batch experiments were performed to measure the profile of various parameters when phosphorus accumulating sludge acclimatized under anaerobic-aerobic operation is fed with malic, lactic, pyruvic, propionic and succinic acids - carboxylic acids that lie in the vicinity of the Glycolytic pathway and the Tricarboxylic acid cycle - two pathways central to the utilization of organics as sources of energy and carbon in most microorganisms. Carbon dioxide gas produced during substrate uptake was also measured. In most cases, intra-cellular carbohydrate reserves are consumed and the NADH2 that is consequently produced requires that there be some subsequent reaction that utilizes it.

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