Intracellular carbon flow in enhanced biological phosphorus accumulating cultures was tracked by using radiolabelled glucose and acetate as substrates. Batch studies using the labelled substrates verified that acetate and glucose were metabolized differently and acetate would be a more effective source of organic material for carbon supplementation. The use of acetate ensures that the greatest percentage of added carbon will be sequestered by phosphorus accumulating species. Carbon flow was also evaluated in bench-scale SBR system by wet chemistry methods to correlate the findings of the batch studies with that of the long-term carbon profiles.

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