The effect of anaerobic stabilization of returned activated sludge in a continuous system on biomass production under batch conditions at various So/Xo ratios was studied. A system without anaerobic sludge stabilization was also operated as a control unit. All experiments were carried out with Toulouse settled wastewater. Biomass growth, substrate removal, oxygen uptake and carbon dioxide production were measured using a quadrupole mass spectrometer (Inficon IQ 200) coupled to a 15-liter fermentor (Biolafitte). The results confirmed that one of the most important parameters in batch cultivation is the initial So/Xo ratio. It was found that anaerobic treatment of activated sludge changed its behaviour during batch cultivation. As a consequence, the observed biomass yield (Yobs) decreased with increasing So/Xo while it remained constant with the sludge from the control unit. In the anaerobic reactor, microorganisms are subject to a physiological shock due to lack of oxygen and food. Under the above conditions, they use ATP as a source of energy. After they are returned to aerobiosis and supplied with exogenous substrate, they rebuild energy reserves at the expense of growth.
Effect of Anaerobic Stabilization of Activated Sludge on its Production under Batch Conditions at Various So/Xo Ratios
P. Chudoba, J. J. Chevalier, J. Chang, B. Capdeville; Effect of Anaerobic Stabilization of Activated Sludge on its Production under Batch Conditions at Various So/Xo Ratios. Water Sci Technol 1 February 1991; 23 (4-6): 917–926. doi: https://doi.org/10.2166/wst.1991.0543
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