Penetration of three kinds of biocides into biofilm formed on the surface of PVC tubes was examined. Penetration depth curves reached a plateau in 2 hours or less. Penetration depth at the steady-state was dependent on external concentrations of the biocides in the fluids. Penetration depth was limited by decomposition of the biocides by biofilm, and the decomposing abilities were maintained even in the killed bacteria.

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