This study is aimed at determining the occurrence of campylobacters in waste water, sewage sludge and in a river system. The selective medium for the isolation of campylobacter consists of blood agar supplemented with the antibiotics vancomycin (10 µg/ml), cephalexin (15 µg/ml), trimethoprim (5 µg/ml), polymycin B (2.5 µg/ml) and rifampicin (5 µg/ml). Raw sewage samples contained about 103 Campylobacter/100 ml while the effluent showed an average concentration of 1.3 × 102/100 ml. Raw sewage from an oxidation pond treatment plant contained an average of 51 Campylobacter/100 ml while none were found in the effluent. No Campylobacter could be found in digested, conditioned sludge. The organism could be detected in 82.1% of river waters examined with the majority showing <10/100 ml. The presence of waterfowl and the faecal contamination from a poultry farm resulted in higher Campylobacter levels. About 50 % of the isolates typed as C. coli were really confirmed as C. jejuni by electrophoretic pattern (whole cell protein profiles).

This content is only available as a PDF.
You do not currently have access to this content.