Astroviruses from three sources, (1) of human origin, adapted to cell culture growth by passage in primary tissue and (2) direct from faeces; (3) of lamb origin, from gut epithelium scrapings were processed to extract the RNA. The first two of these were also compared in their growth on LLCMK2 and Caco-2 cell lines. Virus was successfully isolated direct from faeces in the latter line, though it failed to grow in LLCMK2. Previously adapted virus continued to grow well in both lines. RNA was extracted from the ovine virus in a condition which permitted the production of cDNA grior to cloning, but the RNA from the human astroviruses was more refractory to extraction owing to its aggregation with cell debris.
Growth of Clinical Isolates of Astro Virus in a Cell Line and the Preparation of Viral RNA
A. P. Wyn-Jones, A. J. Herring; Growth of Clinical Isolates of Astro Virus in a Cell Line and the Preparation of Viral RNA. Water Sci Technol 1 July 1991; 24 (2): 285–290. doi: https://doi.org/10.2166/wst.1991.0075
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