F-specific RNA (FRNA) bacteriophages can be assayed in wastewater by simple and rapid methods. Their inactivation by UV radiation follows first-order kinetics and relatively simple formulas can describe the effect of UV absorbance by wastewater and the wavelength-dependent killing efficiency of polychromatic lamps. Because the organisms can be grown in high concentrations, biological calibration of full-scale reactors requires only relatively small volumes of phage culture. Naturally occurring FRNA-phages were more resistant to UV than somatic coliphages, Escherichiacoli and faecal streptococci. The inactivation rate constant was almost equal to that of reoviruses, which underlines the suitability of FRNA-phages as a process indicator for UV inactivation of viruses. A pure culture of MS2 was inactivated at a rate which was almost twice that of naturally occurring FRNA-phages, indicating the necessity of designing reactors for practical applications on field-data rather than laboratory experiments.
F-Specific RNA Bacteriophages as Model Viruses in UV Disinfection of Wastewater
A. H. Havelaar, Th J. Nieuwstad, C. C. E. Meulemans, M. van Olphen; F-Specific RNA Bacteriophages as Model Viruses in UV Disinfection of Wastewater. Water Sci Technol 1 July 1991; 24 (2): 347–352. doi: https://doi.org/10.2166/wst.1991.0088
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