The adsorption mechanism of RNA-F-specific coliphages, Qβ, and poliovirus 1 in the activated sludge process was studied experimentally. The mechanisms of Qβ and poliovirus associated with microbial flocs were attributable to the pH change in the system which was caused by either microbial activity during glucose uptake or the added acids/alkalis. The adsorption mechanisms of them seemed to be similar, and the only difference was the pI level of each other. In addition, Qβwas found to be more tolerant of environmental stress than was the case for poliovirus because of Qβ being stable for a week in a batch activated sludge reactor. By contrast, poliovirus was reduced about 0.8 log in three days in the identical reactor.

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