Two extraction-concentration methods for recovering rotavirus from mussels and oysters are compared. Molluscs are artificially contaminated by a one hour stay in seawater seeded with rotavirus SA 11. Results show that the first procedure using a glycine-NaCl pH 10 solution and an organic flocculation gives quite similar virus recovery from mussels and oysters, whatever the quantities of rotavirus in seawater are. The second procedure using a borate buffer - 3 % beef extract pH 9 and a double precipitation with Polyethylene glycol 6000 leads in most cases to a more efficient virus recovery, based on immunofluorescent foci counts on cover slip cultures of MA 104.

This content is only available as a PDF.
You do not currently have access to this content.