Image analysis was applied to chromosomal aberration tests carried out on Chinese hamster lung cell (CHL). First, chromosomes on pictures which had been judged to be normals or aberrations by experts were analyzed by the authors' image analyzer. 802 chromosomes including 83 aberrations were distinguished by an algorithm which was developed with shape parameters. Consequently, 91.1% of exchanged-type aberrations, 47.4% of broken-type aberrations, and 97.9% of normals were correctly distinguished. And it was concluded that this algorithm could be put into practical detections of exchanged-type aberrations. Subsequently, activity-induced chromosomal aberrations of chlorinated lake water were investigated with the developed algorithm. Activity-induced chromosomal aberrations of chlorinated water depended on the chlorination pH, revealing a pattern of decreasing activity with increasing pH. It was a converse pattern for that of chloroform production.
Research Article|June 01 1992
Detection of Activity-Induced Chromosomal Aberrations Using Image Analysis
Water Sci Technol (1992) 25 (11): 227-234.
S. Ito, H. Sumitomo, Y. Matsuoka; Detection of Activity-Induced Chromosomal Aberrations Using Image Analysis. Water Sci Technol 1 June 1992; 25 (11): 227–234. doi: https://doi.org/10.2166/wst.1992.0296
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