The physico-chemical and mutagenic properties of organic concentrates from the Yodo river water were investigated using XAD resin column method with XAD-2, XAD-4 and XAD-8 under various adsorptive and desorptive conditions. XAD resin concentrates showed to be directly and indirectly frameshift mutagenic. Organic mutagens in the Yodo river water were almost absorbed to XAD-2 resin and then desorbed effectively with CH2Cl2. The physico-chemical properties of these concentrates were acidic and hydrophobic. Additionally organic mutagens which possess basic and hydrophilic properties may contribute to the mutagenicity.

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