Off-flavour compounds produced by algae in freshwater ecosystems were studied for their structure using integrated sensory and spectroscopic methods: mass spectrometry (MS), nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR). Both solvent extraction and thermal desorption were used to isolate and to introduce the compounds into gas chromatography/mass spectrometry (GC/MS) and gas chromatography/Fourier transform infrared (GC/FTIR) systems. Ten ng of a terpenoid compound gave a readable IR spectrum. For 1H NMR studies the compounds were collected directly into NMR solvent by preparative gas chromatography. About 5 µg of a monoterpenoid compound was needed for a reliable 1H NMR spectrum. The mass spectral data indicated that the series of odorous substances detected by GC were terpenoid hydrocarbons and a later eluting series of compounds were sesquiterpene alcohols. In addition, one compound with an intense off-odour (aromatic, nitrogen containing compound) and some tainting carboxylic acids (derived from a starch factory) were detected.
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Research Article|
January 01 1992
Methods for the Identification of Tainting Terpenoids and other Compounds from Algae Available to Purchase
Water Sci Technol (1992) 25 (2): 165–170.
Citation
A. Veijanen, E. Kolehmainen, R. Kauppinen, M. Lahtiperä, J. Paasivirta; Methods for the Identification of Tainting Terpenoids and other Compounds from Algae. Water Sci Technol 1 January 1992; 25 (2): 165–170. doi: https://doi.org/10.2166/wst.1992.0048
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