A chelating resin (Chelex 50-100) and ion-exchange resin (Dowex 50W-X8) were evaluated for removal of heavy metals in toxicity fractionation. Microtox and β-galactosidase activity were employed as toxicity endpoints. The resins were packed into 4 raL glass Pasteur pipettes for use. Chelating resin provided complete removal of toxicity due to polyvalent heavy metal cations (Cd, Cu, Hg, Pb, Zn). Ion-exchange resin was ineffective in removing mercury toxicity. Neither resin provided complete removal of Ag+ toxicity. Toxicity of organic compounds was, at most, partially removed. Performance of the ion-exchange and chelating resins was insensitive to hardness and pH. Based on these results, chelating resin is recommended for heavy metal removal as part of a toxicity fractionation procedure.

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