The metabolic pathway of the phenol degradation in Pseudomonasputida BH was amplified by introducing the recombinant plasmid containing catechol 2,3 oxygenase gene isolated fron the chromosome of BH. This strain could degrade phenol and grow much faster than the wild strain at the phenol concentration of 100mg/L. This strain seems to accelerate the phenol removal rate if it is applied to the treatment of wastewater containing phenol.

Phenol degradation, wastewater treatment, Pseudomonasputida BH, amplification of metabolic pathway, recombinant plasmid, catechol 2,3 oxygenase, rate-determining step
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© IWA Publishing 1992
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