This study developed a reverse transcriptase-polymerase chain reaction (RT-PCR) detection system for enteric viruses in sample concentrates obtained by conventional filter adsorption-elution methods. One liter beef extract (BE)-glycine (G) eluant seeded with poliovirus 1 and hepatitis A virus (HAV) was used as a model system and the eluant further processed for RT-PCR compatibility. Sample concentration and purification procedures consisted of polyethylene glycol (PEG) precipitation, Pro-Cipitate (Affinity Technology, New Brunswick, NJ) precipitation, a second PEG precipitation, spin-column chromatography, and ultrafiltration. Sample volumes are reduced from 1 L to 20-40 µL and purified sufficiently for viral detection by RT-PCR. As little as 3 PFU of poliovirus 1 in an initial 1 L eluate were detected by RT-PCR.
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Research Article| February 01 1993
Development of PCR Methods for Enteric Virus Detection in Water
Water Sci Technol (1993) 27 (3-4): 211–218.
K. J. Schwab, R. De Leon, M. D. Sobsey; Development of PCR Methods for Enteric Virus Detection in Water. Water Sci Technol 1 February 1993; 27 (3-4): 211–218. doi: https://doi.org/10.2166/wst.1993.0348
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