Oyster samples processed by adsorption-elution-precipitation were seeded with poliovirus 1 and HAV, and cleaned and concentrated by Freon extraction (2X), PEG precipitation and chloroform extraction. Freon extraction resulted in recoveries of 63-76% for polio and 42-52% for HAV. PEG precipitation/chloroform extraction gave recoveries of 47-50% for polio and 15-19% for HAV. Treated extracts inhibited RT-PCR at 10−2 dilutions. Inhibitors were removed by treatment with the cationic detergent CTAB or Pro-Cipitate/UF adsorption-elution-concentration. Both treatments resulted in samples on which direct RT-PCR was possible. The CTAB procedure was able to detect 78 pfu of polio and 295 pfu of HAV. The Pro-Cipitate procedure was able to detect 70 pfu polio and 2.1×103 pfu HAV.
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Research Article| February 01 1993
Application of RT-PCR for the Detection of Enteric Viruses in Oysters
Water Sci Technol (1993) 27 (3-4): 49–53.
L. A. Jaykus, R. De Leon, M. D. Sobsey; Application of RT-PCR for the Detection of Enteric Viruses in Oysters. Water Sci Technol 1 February 1993; 27 (3-4): 49–53. doi: https://doi.org/10.2166/wst.1993.0320
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