This study was designed to develop a modification of A-ELISA performed in microtitre plates. Nylon filters have been utilized successfully as a solid phase for the performance of A-ELISA. The use of nylon filters resulted in lower background than nitro-cellulose and paper filters, indicating their suitability as a solid phase for developing A-ELISA. With enteric viruses, human rotaviruses and MS-2 coliphage, negative results were obtained, suggesting high specificity of the developed technique for poliovirus 1. The sensitivity of the developed A-ELISA has been shown to be at least one order of magnitude greater than ordinary ELISA. A positive result with the nylon A-ELISA can be obtained with samples containing 100-1000 pfu/ml of poliovirus. Up to date methods used for detecting viruses in water are elaborate, time consuming and costly. Applying the nylon A-ELISA may overcome some of these disadvantages.
Research Article|April 01 1993
A Nylon Filter A-ELISA for Detecting Viruses in Water
Abidelfatah M. Nasser
Water Sci Technol (1993) 27 (7-8): 135-141.
Abidelfatah M. Nasser, Yehudit Elkana, Leon Goldstein; A Nylon Filter A-ELISA for Detecting Viruses in Water. Water Sci Technol 1 April 1993; 27 (7-8): 135–141. doi: https://doi.org/10.2166/wst.1993.0544
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