The present work describes an adaptation of the regionalized sensitivity analysis based on Monte Carlo simulations for the parameter estimation and sensitivity analysis of an activated sludge model. The procedure described should be used when observed data are available for the model calibration, which is nevertheless still limited by the problems inherent to activated sludge models (uncertainty and lack of identifiability). The selection between good and bad performance of the model is judged based on the Coefficient of Determination. The application of the procedure to an 11-parameter 4-state dynamic activated sludge model used for operational control was considered satisfactory. The method is simple and yet robust, and the analyst's involvement in the interpretation of the results and decision upon the next steps to be taken increases its controllability.

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