A series of nitrification kinetic tests was carried out with both suspended and fixed biomasses originated from a combined activated sludge system with a plastic support for biomass growth introduced into the aeration tank (Experimental combined unit - ECU). The results of these batch tests were compared with kinetics calculated from continuously run pilot plant. It was concluded that nitrification kinetics of both suspended and attached biomasses were similar and, consequently, it was assumed that the percentage of autotrophs is the same in both biomasses. The kinetics determined in both batch and continuous systems were comparable. Kinetic constants measured for suspended biomass of the ECU were higher than those reported by the literature, which was attributed to an overaeration of the ECU and an energetic mixing

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