Two viability substrates, ChemChrome B and 5-cyano-2,3,4-tolyl-tetrazolium chloride (CTC) were compared with the traditional pour plate method for the enumeration of bacteria in potable water samples. ChemChrome B stained cells were counted, on a membrane, using a novel laser scanning device whereas CTC stained cells were counted using epifluorescence microscopy. A significantly higher number of bacteria were detected using the viability assays than by culture, which highlights the inefficiency of current culture techniques. Results with ChemChrome B illustrate that the use of a laser scanning device provides an efficient method for the enumeration of fluorescently labelled microorganisms.
Development of a rapid method for the enumeration of bacteria in potable water
D. T. Reynolds, E. J. Fricker, D. Purdy, C. R. Fricker; Development of a rapid method for the enumeration of bacteria in potable water. Water Sci Technol 1 June 1997; 35 (11-12): 433–436. doi: https://doi.org/10.2166/wst.1997.0773
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