A method has been developed for the detection of infectious human rotavirus (HRV), based on infection of MA104 and CaCo-2 cell monolayers and ulterior flow cytometry. The sensitivity of the flow cytometry procedure for the cell-adapted HRV enabled the detection of 200 and 2 MPNCU in MA104 and CaCo-2 cells, respectively. Flow cytometry performed five days after infection of CaCo-2 enabled the detection of naturally occurring wild-type HRV in faecal samples and concentrated water samples.
Evaluation of flow-cytometry for the monitoring of infectious human rotavirus in water
F. X. Abad, A. Bosch, J. Comas, D. Villalba, R. M. Pintó; Evaluation of flow-cytometry for the monitoring of infectious human rotavirus in water. Water Sci Technol 1 June 1997; 35 (11-12): 451–453. doi: https://doi.org/10.2166/wst.1997.0776
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