In this study, we tried to evaluate the physiological state of E. coli exposed to chlorine by using non-selective media and media supplemented with different concentrations of selective agent. A new kinetic model – transportation equation model – was developed based on the physiological change of bacteria for describing the injury process of E. coli induced by chlorine and applied to the experimental results. Conclusions can be summarised as: 1) physiological state of E. coli exposed to chlorine can be determined by using different selective media; 2) the tolerance distribution of E. coli to sodium deoxycholate shifted to a lower concentration with contact time; 3) the transportation equation model presented in this study agrees with experimental results; and 4) the transportation equation model is applicable to analyse the injury process of E. coli in chlorination.

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