The diversity of autotrophic ammonia-oxidizing bacteria (AOB) of the b-subdivision of the class Proteobacteria was investigated in a laboratory-scale denitrification-nitrification bioreactor (DNB) treating a synthetic waste stream. 16S ribosomal RNA (rRNA) gene sequences were amplified from DNA extracted from the oxic DNB sludge. Comparative analysis of the rRNA sequences revealed considerable diversity among the AOB-like sequences. The majority of sequences recovered were related to Nitrosomonas spp. but a smaller number of Nitrosospira-like sequences were obtained. Since different AOB may have different kinetic properties the high diversity of AOB, even in a simple laboratory biotreater treating a simple waste stream, has important implications for the operation of nitrifying wastewater treatment processes.
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Research Article|
February 01 1998
Molecular microbial ecology of nitrification in an activated sludge process treating refinery wastewater
S. J. Ballinger;
S. J. Ballinger
*Fossil Fuels and Environmental Geochemistry Postgraduate Institute, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
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I. M. Head;
I. M. Head
*Fossil Fuels and Environmental Geochemistry Postgraduate Institute, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
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T. P. Curtis;
T. P. Curtis
**Department of Civil Engineering, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
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A. R. Godley
A. R. Godley
***Shell Research Ltd, Thornton Research Centre, P.O. Box 1, Chester CH1 3SH, UK
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Water Sci Technol (1998) 37 (4-5): 105–108.
Citation
S. J. Ballinger, I. M. Head, T. P. Curtis, A. R. Godley; Molecular microbial ecology of nitrification in an activated sludge process treating refinery wastewater. Water Sci Technol 1 February 1998; 37 (4-5): 105–108. doi: https://doi.org/10.2166/wst.1998.0594
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