The biotransformation of 3-chlorobenzoate (3CB) by attached cells of Pseudomonas sp. strain B13 was studied in percolation columns operated at various flow rates and biomass contents. Steady-state residual effluent concentrations were compared with predictions from spreadsheet models describing the combined action of microbial and mass transfer kinetics. The effluent concentrations were successfully predicted above a critical ratio of flow rate and biomass. Below this critical point the steady-state residual effluent concentrations were higher than predicted and this deviation increased with decreasing flow rate:biomass ratios. The results are discussed in the light of convection-diffusion processes limiting the transport of both substrate and oxygen to the attached cells.
Anomalies in the transformation of 3-chlorobenzoate in percolation columns with Pseudomonas sp. strain b13
Marijke E. Tros, Gosse Schraa, Alexander J.B. Zehnder, Tom N.P. Bosma; Anomalies in the transformation of 3-chlorobenzoate in percolation columns with Pseudomonas sp. strain b13. Water Sci Technol 1 April 1998; 37 (8): 89–96. doi: https://doi.org/10.2166/wst.1998.0312
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