Four anaerobic-aerobic sequencing batch reactors were operated with either acetate or glucose as the main carbon sources under phosphorus poor or rich conditions. Limited phosphate loading may suppress the development of polyphosphate accumulating organisms (PAOs) leading to establishment of microbial communities without polyphosphate accumulation. These microbial communities were dominated by glycogen accumulating organisms (GAOs). PAO communities were established under the P rich loading conditions. Both PAO and GAO sludges clearly show that acetate absorption was accompanied by glycolysis with subsequent accumulation of PHAs. Glucose was anaerobically converted to PHAs and glycogen. Glycolysis in PHA synthesis was indicated by the presence of a PHA component polymer, 3-hydroxyvalerate (3HV). It is suspected that synthesis of 3HV is via formation of the intermediate metabolite Propionyl-CoA which consumes reducing power and enables microorganisms to maintain intracellular reduction oxidation potential. When glucose was injected, acetate and propionate were detected in the bulk solution suggesting that fermentation may have taken place. Respiratory quinone analyses and fluorescent in situ hybridization with an RNA targeted oligonucleotide probe revealed that there was no significant difference in microbial communities among the sludges tested.
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Research Article|
March 01 1999
Metabolism of enhanced biological phosphorus removal and non-enhanced biological phosphorus removal sludge with acetate and glucose as carbon source
I. M. Sudiana;
I. M. Sudiana
*Department of Urban Engineering The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 153, Japan
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T. Mino;
T. Mino
*Department of Urban Engineering The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 153, Japan
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H. Satoh;
H. Satoh
*Department of Urban Engineering The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 153, Japan
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K. Nakamura;
K. Nakamura
**National Institute of Human Science and Technology Tsukuba, Agency of Human Science and Technology, 1,1 Higashi, Tsukuba, Ibaraki 305, Japan
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T. Matsuo
T. Matsuo
*Department of Urban Engineering The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 153, Japan
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Water Sci Technol (1999) 39 (6): 29–35.
Citation
I. M. Sudiana, T. Mino, H. Satoh, K. Nakamura, T. Matsuo; Metabolism of enhanced biological phosphorus removal and non-enhanced biological phosphorus removal sludge with acetate and glucose as carbon source. Water Sci Technol 1 March 1999; 39 (6): 29–35. doi: https://doi.org/10.2166/wst.1999.0253
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