This paper describes several techniques and methods for sludge characterization which are recently developed and used in our ongoing research on biological phosphorus removal (BPR): (i) chemical measurement of the poly-hydroxy-alkanoate (PHA) content of the biomass, (ii) bioassay for glycogen determination in BPR systems, (iii) determination of the fraction of denitrifying phosphorus accumulating organisms (DPAOs) in P-removing sludge, (iv) determination of the aerobic and anoxic ATP/NADH2 ratio (δ value) in BPR systems, and (v) determination of the glycogen accumulating organisms (GAOs) / phosphorus accumulating organisms (PAOs) ratio in activated sludge.
Innovative methods for sludge characterization in biological phosphorus removal systems
Damir Brdjanovic, Mark C. M. van Loosdrecht, Christine M. Hooijmans, Takashi Mino, Guy J. Alaerts, Joseph J. Heijnen; Innovative methods for sludge characterization in biological phosphorus removal systems. Water Sci Technol 1 March 1999; 39 (6): 37–43. doi: https://doi.org/10.2166/wst.1999.0255
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