This article defines some quantitative parameters for describing the structure of a biofilm. The parameters can be calculated from a two-dimensional cross-sectional image on a plane parallel to the substratum within an in situ biofilm. Such images can be acquired using a confocal scanning laser microscope (CSLM). The parameters will eventually be used for eliciting relationships between the biofilm's structure and its biochemical function, and for computer model evaluation. The results shown here indicate that the structural parameters appear to be reaching steady-state conditions as the biofilm grows to a steady state.

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