A continuous decolorisation system is proposed as a pretreatment or post-treatment step in an integrated treatment system, combining the use of fungi and anaerobic bacteria. The system consisted of a pulsing packed-bed bioreactor operating with white-rot fungi. This methodology was effective to achieve high decolorisation of a coloured compound, the polyanthraquinone dye Poly R-478, which was degraded up to percentages exceeding 70%. Moreover, a stable operation was maintained for an extended period of time (more than 100 days). The proposed system deserves consideration as a combined bacterial-fungal system to be applied in decolorisation and possibly detoxification of industrial coloured effluents.

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