Mature male medaka were continuously exposed to 0.005, 0.0–5 or 1.0 ppb of estradiol-17β (E2 or 0.1, 10 or 100 ppb of p-nonylphenol (NP) or bis-phenol-A (BPA). Female-specific proteins (Fsp) were induced in medaka exposed to 0.005 ppb of E2, 0.1 ppb of NP, or 10 ppb of BPA. Concentrations of 0.005 pbb of E2 and 0.1 ppb of NP corresponded to concentrations of these chemicals detected in river water in Japan. The abilities of the 3 chemicals to induce Fsp were E2> NP> BPA. Embryonic medaka were exposed to E2, NP and BPA under conditions of static-renewal for 200–230 days until pre-maturity. Survival ratios of medaka exposed to E2 and NP declined in concentrations more than 25 ppb and 50 ppb, respectively. The groups of medaka exposed to E2 had individuals with testis-ova or abnormal gonad. There was no male in exposure to 1.0 ppb E2. When exposed to 100 ppb of NP or BPA, abnormal gonad was also detected. Abnormal anal fin (female-like) was observed in male exposed to 100 ppb of NP. The LC50 values for each of the 3 chemicals were much higher than the concentrations detected in water in the environment—the 3 chemicals were considered to have no lethal effect on medaka in aquatic environments. However, exposures to E2 or NP at environmental concentrations induced Fsp. BPA also had the ability to affect medaka as an environmental estrogen, although its extrogenic activity was weaker than that of E2 or NP.
Skip Nav Destination
Article navigation
Research Article|
January 01 2001
Estrogenic influences of estradiol-17b, p-nonylphenol and bis-phenol-A on Japanese Medaka (Oryzias latipes) at detected environmental concentrations Available to Purchase
A. Tabata;
A. Tabata
1Department of Urban Environmental Engineering, Faculty of Engineering, Hokkaido University, N-13, W-8, Kita-ku, Sapporo, 060–8626, Japan
Search for other works by this author on:
S. Kashiwada;
S. Kashiwada
2Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd., Oh-igawa 1334–5, Shizuoka, 421–0212, Japan
3To whom correspondence should be addressed
Search for other works by this author on:
Y. Ohnishi;
Y. Ohnishi
2Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd., Oh-igawa 1334–5, Shizuoka, 421–0212, Japan
Search for other works by this author on:
H. Ishikawa;
H. Ishikawa
2Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd., Oh-igawa 1334–5, Shizuoka, 421–0212, Japan
Search for other works by this author on:
N. Miyamoto;
N. Miyamoto
2Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd., Oh-igawa 1334–5, Shizuoka, 421–0212, Japan
Search for other works by this author on:
M. Itoh;
M. Itoh
2Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd., Oh-igawa 1334–5, Shizuoka, 421–0212, Japan
Search for other works by this author on:
Y. Magara
Y. Magara
1Department of Urban Environmental Engineering, Faculty of Engineering, Hokkaido University, N-13, W-8, Kita-ku, Sapporo, 060–8626, Japan
Search for other works by this author on:
Water Sci Technol (2001) 43 (2): 109–116.
Citation
A. Tabata, S. Kashiwada, Y. Ohnishi, H. Ishikawa, N. Miyamoto, M. Itoh, Y. Magara; Estrogenic influences of estradiol-17b, p-nonylphenol and bis-phenol-A on Japanese Medaka (Oryzias latipes) at detected environmental concentrations. Water Sci Technol 1 January 2001; 43 (2): 109–116. doi: https://doi.org/10.2166/wst.2001.0079
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
eBook
Pay-Per-View Access
$38.00