The phenol content of a petroleum refinery wastewater was reduced below the discharge limit following treatment with horseradish peroxidase and H2O2. Approximately 58% of COD, 78% of BOD5, and 95% of toxicity were removed along with the phenols. As a result of treatment, phenols were transformed into less biodegradable compounds which could be removed by subsequent coagulation and precipitation. Optimization of the peroxide concentration led to 20% enzyme savings. The use of PEG and chitosan as protective additives resulted in 4 and 25-fold reductions in enzyme requirements, respectively. Phenol removal did not appear to be adversely affected by the presence of other hydrocarbons that are frequently present in refinery wastewaters.
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Research Article| January 01 2001
Peroxidase-catalyzed removal of phenols from a petroleum refinery wastewater
Water Sci Technol (2001) 43 (2): 253–260.
M. Wagner, J. A. Nicell; Peroxidase-catalyzed removal of phenols from a petroleum refinery wastewater. Water Sci Technol 1 January 2001; 43 (2): 253–260. doi: https://doi.org/10.2166/wst.2001.0097
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